In mapping a large plasmid you notice that the restriction enzyme HaeII yields three bands, Pst I yields five bands and HaeII+PstI double digestion gives rise to six bands
A) What is the inconsistency of these data and what are two possible explanations?
B) How could you distinguish between these possibilities from scrutiny of the gel electrophoresis results?
C) You want to ligate the largest fragment generated by PstI with the largest fragment generated by HaeII. However the ends that are generated by the two enzymes are not complementary. What would you need to do in order to carry out the ligation?
