1) Describe how cDNA is produced, clearly indicating the relationship between a gene, an mRNA, and a cDNA?
2) What is an EST? How is an EST different from a genomic sequence? How is an EST different from a cDNA sequence? What is the EST database? What is a UniGene sequence (the Unigene database if you prefer)?
3)Describe a microarray and how it works? Indicate what is fixed to a support and what is used to probe the microarray. How does a microarray differ from a Northern blot?