Read the Lab 12 procedures and watch the online Hardy-Weinberg video (https://youtu.be/xPkOAnK20kw and posted on BlackBoard), then complete this assignment prior to lab.
- The Hardy-Weinberg Theorem states
- What are the five key assumptions that are necessary for the H-W Theorem to be valid?
- Write the Hardy-Weinberg equation:
- Dominant allele R has a frequency (p) of 0.45 in a particular gene pool. Calculate the following showing all your work and using the proper variables for each value (e.g. p, q, p2, q2, 2pg).
- The frequency of allele r in that same gene pool?
- The proportion of the population that has the genotype RR.
- The proportion of the population that has the genotype Rr.
- The proportion of the population that has the genotype rr.
- If 17% of a population displays the recessive trait for Disease B, what are the frequencies of the recessive allele b and the dominant allele B in the gene pool?
- You perform an experiment where you allow a large population of fruit flies to mate randomly. The parental generation had 30% homozygous recessive genotypes. The F1 generation consisted of 100 flies, 40 of which displayed the recessive trait. Calculate the expected values for each phenotype assuming Hardy-Weinberg equilibrium, then fill in the table below and use the Chi-Square test instructions document (posted online) to compare your calculated X2 value with the tabulated X2 value for a P-value of 0.05.
|# of dominant phenotype individuals||# of recessive phenotype individuals|
|Observed value (o)|
|Expected value (e)|
|Deviation (o e) = d|
|Calculated Chi-square (X2) = ?d2/e|
|Degrees of Freedom|
|Tabulated X2 value at P=0.05
(from X2 instructions document)
- According to your analysis above, are the observed proportion of genotypes in the F1 generation the same, or significantly different, than those expected according to the H-W theorem?
- If you allowed your F1 generation to mate, what would you expect the frequency