Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase. Describe how to make the following reaction:
5mM MgCl2
1x buffer
1mM dNTP mix
10ng of primer 1
10ng of primer 2
1ug of Rna
0.25 units of reverse transcriptase
Final reaction 20ul
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