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Discuss how the five cap is formed in eukarytotic mrnas

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Assignment

Instructions:

1. Discuss the function in detail of each of the following RNA polymerase II transcription factors.

TFIIB
TFIIE
TFIIF
TFIIH

2. What aIDitional role does one of the subunits of TFIIH have during the initiation phase that involves other protein kinases? What is the end result?

3. Discuss in detail how the 5′ cap is formed in eukarytotic mRNAs.

4. What makes the 3′ end of eukaryotic mRNAs unique? Describe how this is aIDed.

5. What are the five snRNAs involved in splicing reaction, and describe briefly how they work to assemble spliceosomes.

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