Vitamin D was aIDed to reaction vessels at different concentrations containing its receptor protein (at a fixed concentration). After equilibrium was reached, the amount of Vitamin D bound to the protein was determined. Here are the results determined
Vit.D nmol/liter RL concentration
Draw a graph to demonstrate the amount of vitamin D bound as a function of its concentration. Use the double reciprocal method to determine values for the dissociation constant and the total amount of receptor present?