Briefly describe the series of enzymatic treatments

You want to make an antibody against a C. elegans nematode protein that the DNA shown in Figure A encodes. To do this, you will clone the coding sequence into a bacterial expression plasmid, overexpress the protein, purify it, and inject it into a mouse to stimulate the production of antibodies against the protein.

A.         You use polymerase chain reaction (PCR) amplification to amplify the DNA corresponding to the 1800 base pairs of coding sequence; this coding sequence lies between the two flanking sequence shown in Figure A. You will then insert the PCR product into the BamHI site of the expression plasmid shown in Figure B. What are the sequences of the two oligonucleotide primers that will allow you to amplify the DNA by PCR and insert it into the BamHI site? (Remember to indicate the 5′ and 3′ ends of the primers.)

B.         Briefly describe the series of enzymatic treatments you will use to create the expression plasmid before transferring it into bacterial cells.

C.         Several weeks after you inject a mouse with your purified protein, you sample its blood serum, which contains antibodies. You know that the mRNA corresponding to your protein is found in gonad cells but not in gut cells. To test whether the mouse has made antibodies against your protein, you isolate protein from gonad cells and from gut cells and perform a Western blot (also known as an immunoblot) with the mouse serum and a fluorescent second antibody that binds mouse antibodies. Your result is shown in Figure Did the mouse make antibodies against your protein? Does the serum specifically recognize only one nematode protein? (Illustrate your written answers by labeling the blot.)

 

1543_Screen Shot 2015-11-04 at 12.33.52 AM.png

                                   2300_Screen Shot 2015-11-04 at 12.33.42 AM.png

 

2146_Screen Shot 2015-11-04 at 12.33.28 AM.png

Order a similar paper and get 15% discount. Use the coupon code GILB

Dr. Padma Myers
Dr. Padma Myers
98% Success Rate
Read More
“Hello, I deliver nursing papers on time following instructions from the client. My primary goal is customer satisfaction. Welcome for plagiarism free papers”
Stern Frea
Stern Frea
98% Success Rate
Read More
Hi! I am an English Language and Literature graduate; I have written many academic essays, including argumentative essays, research papers, and literary analysis.
Dr. Ishid Elsa
Dr. Ishid Elsa
98% Success Rate
Read More
"Hi, count on me to deliver quality papers that meet your expectations. I write well researched papers in the fields of nursing and medicine".
Dr. Paul P. Klug
Dr. Paul P. Klug
99% Success Rate
Read More
"A top writer with proven reliability and experience. I have a 99% success rate, overall rating of 10. Hire me for quality custom written nursing papers. Thank you"

How Our Essay Writing Service Works

Tell Us Your Requirements

Fill out order details and instructions, then upload any files or additional materials if needed. Then, confirm your order by clicking “Place an Order.”

Make your payment

Your payment is processed by a secure system. We accept Mastercard, Visa, Amex, and Discover. We don’t share any informati.on with third parties

The Writing Process

You can communicate with your writer. Clarify or track order with our customer support team. Upload all the necessary files for the writer to use.

Download your paper

Check your paper on your client profile. If it meets your requirements, approve and download. If any changes are needed, request a revision to be done.

Recent Questions

Stay In Touch!

Leave your email and get discount promo codes and the best essay samples from our writers!