Approximate how numerous clones you will need to screen

Q. Suppose a mammalian genome (4×10^9bp in size) is digested with Hind III, and you are cloning a unique fragment of 2kb.

A. Approximate how numerous clones you will need to screen?

B. If you first size select the genomic DNA, realizing a 10-fold purification, and clone that DNA in a plasmid, now how many clones will you need to screen?

C. If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen?

D. If you screen 5,000 colonies on a 15cm dish, how various dishes will you require to find a clone of interest? “

Order a similar paper and get 15% discount. Use the coupon code GILB

Dr. Padma Myers
Dr. Padma Myers
98% Success Rate
Read More
“Hello, I deliver nursing papers on time following instructions from the client. My primary goal is customer satisfaction. Welcome for plagiarism free papers”
Stern Frea
Stern Frea
98% Success Rate
Read More
Hi! I am an English Language and Literature graduate; I have written many academic essays, including argumentative essays, research papers, and literary analysis.
Dr. Ishid Elsa
Dr. Ishid Elsa
98% Success Rate
Read More
"Hi, count on me to deliver quality papers that meet your expectations. I write well researched papers in the fields of nursing and medicine".
Dr. Paul P. Klug
Dr. Paul P. Klug
99% Success Rate
Read More
"A top writer with proven reliability and experience. I have a 99% success rate, overall rating of 10. Hire me for quality custom written nursing papers. Thank you"

How Our Essay Writing Service Works

Tell Us Your Requirements

Fill out order details and instructions, then upload any files or additional materials if needed. Then, confirm your order by clicking “Place an Order.”

Make your payment

Your payment is processed by a secure system. We accept Mastercard, Visa, Amex, and Discover. We don’t share any informati.on with third parties

The Writing Process

You can communicate with your writer. Clarify or track order with our customer support team. Upload all the necessary files for the writer to use.

Download your paper

Check your paper on your client profile. If it meets your requirements, approve and download. If any changes are needed, request a revision to be done.

Recent Questions

Stay In Touch!

Leave your email and get discount promo codes and the best essay samples from our writers!