Question: A 2.5 mg/ml stock solution was diluted as described in the table below and then the absorbance of each dilution was determined and presented in the table below. Using this information, what is the concentration in mg/ml (to two decimal places) of an unknown sample with an absorbance of 0.41?(Hint: You will have to calculate the concentration in each diluted tube)
Volume of 2.5 mg/ml Stock Volume of Buffer Absorbtion
5ml 0ml 1.0
4ml 1ml 0.8
3ml 2ml 0.6
2ml 3ml 0.4
1ml 4ml 0.2
0ml 5ml 0.0
